Reconciliation rumors started swirling when photos emerged last week of Courteney Cox and David Arquette at Disneyworld with their six-year-old daughter, Coco.
But on Ryan Seacrest's radio show April 11, Arquette, 39, shot down stories that the separated Scream stars were reunited.
"We're not together," said the funnyman. "She's my best friend, and I love her with all my heart."
Calling their relationship "open and honest," Arquette said his estranged wife, 46, told him about her April 1 trip to St. Barts with Cougar Town costar Josh Hopkins.
"We have 15 years together of love and friendship," he said. "We're in a better place right now then we were."
Arquette acknowledged that their relationship hadn't been as strong when the two of them were filming Scream 4 (opening Friday) last summer.
"We were together, but there were talks of separation," he said. "We took a moment to look at our relationship -- see if we were happy and ask some questions."
After announcing their split last October, Arquette admits he used the time apart in a "bad way."
"I used it to act out and shake up my world because I was heartbroken," he says. "I dealt with it in the way I had to. I went where I had to go to look at myself."
In January, the actor entered rehab, and recently announced he's been sober for 100 days. And a head-on car collision in March gave him additional insight.
"I was knocked out and had this crazy dream with spiritual women around me," he says. "It gave me perspective and reminded me to take it slow, that life is short."