Reaching in for a real grab of flesh, Hudson was momentarily taken aback by her pal's forward nature, opening her mouth in shock as the duo stood before E!'s Ryan Seacrest, ready for a quick red carpet interview.
"I'm sorry, I had to do it!" Aniston quipped while Seacreast stepped forward.
"What did you just ask me to do?" a cheeky Seacrest asked.
"I didn't say you had to do anything, that was what I had to do," she said, laughing. "Irresistable," she added, giving her pal another quick pat on the backside.
Aniston went on to join her date for the evening, fiance Justin Theroux, and remained in cheery spirits despite losing out on her Best Actress in a Drama award to Julianne Moore.
And as it turned out, Aniston's wasn't the only attention Hudson's ass got on Golden Globe night! See stunning pics of Aniston with Theroux on the red carpet.
During a commercial break in the show, the Wish I Was Here actress received a playful poke in the butt from Matthew McConaughey as she walked past his table.
And later in the evening, while leaving the Golden Globes Fox After Party sponsored by Patron, Hudson received a friendly pat on the backside from Paul Rudd, as she waited for her car to arrive!
Sign up now for the Us Weekly newsletter to get breaking celebrity news, hot pics and more delivered straight to your inbox!