Madonna Performs “Same Love” With Macklemore, Ryan Lewis, Mary Lambert: Watch Queen Latifah Officiate 33 Weddings During the Performance

A legendary collaboration took place at the 2014 Grammy Awards, which aired live from the Staples Center in Los Angeles on Sunday, Jan. 26. Pop icon Madonna took the stage with rapper Macklemore & Ryan Lewis and featured vocalist Mary Lambert to sing the marriage-equality anthem "Same Love."

But that wasn't the only historic moment from the performance. Performer, actress, and talk show host Queen Latifah officiated the weddings of 33 couples, including Ryan Lewis' sister Laura. 

Macklemore madonna
Madonna, Mary Lambert, and Macklemore perform "Same Love" at the 2014 Grammy Awards. Kevin Winter/WireImage.com

The emotional moment is reminiscent of the performance at the 2013 VMAs when American Idol alum Jennifer Hudson also joined the Grammy-nominated stars on stage for a duet on the tune. 

Madonna also treated the crowd to an excerpt of her hit "Open Your Heart" with a featured choir to back the legend up as the newly-wed couples danced joyously in the aisles. 

madonna queen latifah
Madonna sings "Open Your Heart" as Queen Latifah officiates 33 weddings at the 2014 Grammys. Kevork Djansezian/Getty

As the married couples exited the auditorium, the crowd jumped to their feet, and many, including Keith Urban, wiped back tears. 

Tell Us: What did you think of Madonna's duet with Macklemore & Ryan Lewis?

Sign up now for the Us Weekly newsletter to get breaking celebrity news, hot pics and more delivered straight to your inbox!

Want stories like these delivered straight to your phone? Download the Us Weekly iPhone app now!