Credit: Frazer Harrison/Getty Images

Looks like there's no hope for a reconciliation between George Lopez and Ann Serrano, married since 1993.

In documents obtained by RadarOnline, Serrano cited "irreconcilable differences" when she filed a divorce petition in L.A. Tuesday afternoon.

Represented by attorney Laura Wasser (who currently reps Christina Aguilera as well), Serrano is seeking spousal support and primary physical custody of their 14-year-old daughter, Mayan.

When the couple announced their split in September, a rep for Lopez, 49, told that "the decision is amicable and by mutual agreement. They remain dedicated parents, and committed partners in business and their philanthropic organization, The Lopez Foundation."