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Looks like there's no hope for a reconciliation between George Lopez and Ann Serrano, married since 1993.

In documents obtained by RadarOnline, Serrano cited "irreconcilable differences" when she filed a divorce petition in L.A. Tuesday afternoon.

Represented by attorney Laura Wasser (who currently reps Christina Aguilera as well), Serrano is seeking spousal support and primary physical custody of their 14-year-old daughter, Mayan.

When the couple announced their split in September, a rep for Lopez, 49, told UsMagazine.com that "the decision is amicable and by mutual agreement. They remain dedicated parents, and committed partners in business and their philanthropic organization, The Lopez Foundation."