"I would say I've always been confused with my gender identity since I've been this big," Jenner told Sawyer immediately, holding his hand up a few feet from the floor. "Let's give him the soul of a female, and see how he deals with that... I hate that term: 'Girl stuck in a guy's body.'"
Often teary throughout the interview, Jenner, 65, slowly unraveled his ponytail as Sawyer asked him: "Are you a woman?"
"Yeah, why not, huh. We're talking about all this stuff. Let's take the damn ponytail out."
"Yes," replied Jenner. "For all intents and purposes, I am a woman. People look at me differently. They see me as this 'macho male.'"
He elaborated: "That female side of me is part of me. That's who I am. I was not genetically born that way. Umm, as of now I have all the male parts and all that kind of stuff. In a lot of ways, we're different, but we still identify as female."
Sawyer explained that this is the final interview Jenner will ever conduct as a male. Watch the emotional moment above.
For more details on his kids' reactions, who found out first, plus whether Kris Jenner had a comment, check out Us Weekly's live blog from the Bruce Jenner Special with Diane Sawyer on ABC.